7MEV
Human Apex/Ref1 monomer with C138A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER TURBO X-RAY SOURCE |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-16 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.242, 74.447, 45.279 |
| Unit cell angles | 90.00, 111.61, 90.00 |
Refinement procedure
| Resolution | 21.377 - 1.600 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7mcr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data reduction software | XPREP |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.377 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.015 | 0.159 |
| Rmeas | 0.021 | 0.225 |
| Rpim | 0.015 | 0.159 |
| Number of reflections | 31841 | 3081 |
| <I/σ(I)> | 33.19 | 4.72 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 1.9 | 1.6 |
| CC(1/2) | 1.000 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 20 %w/v PEG 3350, 200 mM Sodium nitrate |
