7M7N
Human DNA Pol eta with 2'-FA-ended primer and dAMPNPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.444, 98.444, 82.105 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.310 - 1.310 |
R-factor | 0.1863 |
Rwork | 0.184 |
R-free | 0.21030 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4ecq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.870 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.330 |
High resolution limit [Å] | 1.310 | 3.550 | 1.310 |
Rmerge | 0.057 | 0.026 | 0.689 |
Total number of observations | 413501 | ||
Number of reflections | 104816 | 5271 | 4978 |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 97.1 | 95.6 | 92.6 |
Redundancy | 3.9 | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 295 | 15% PEG 2000 MME, 100 mM MES |