7M4M

Crystal structure of RBR E3 ligase RNF216 with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953724
Spacegroup name	P 41 21 2
Unit cell lengths	81.871, 81.871, 248.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 2.390
R-factor	0.2049
Rwork	0.200
R-free	0.24680
Structure solution method	SAD
Starting model (for MR)	RNF216/ubiquitin
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	2.732
High resolution limit [Å]	2.387	2.387
Rmerge	0.126	2.276
Rmeas	0.132	2.378
Rpim	0.037	0.678
Number of reflections	18242	913
<I/σ(I)>	13.8	1.4
Completeness [%]	52.6	8.1
Redundancy	13	12.1
CC(1/2)	0.998	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15% PEG 6000, 0.18 M NaCl, 0.09 M Na HEPES pH7.0, 0.1 M KCl