7M2I

Structural Snapshots of Intermediates in the Gating of a K+ Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-06-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03
Spacegroup name	I 4
Unit cell lengths	155.250, 155.250, 74.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.812 - 2.695
R-factor	0.2305
Rwork	0.228
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.850
High resolution limit [Å]	2.690	2.720
Number of reflections	24336	3166
<I/σ(I)>	8.1
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.990	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate