7M10

PHF2 PHD Domain Complexed with Peptide From N-terminus of VRK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	34.240, 63.717, 103.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.500 - 1.150
R-factor	0.157
Rwork	0.157
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kqi
RMSD bond length	0.004
RMSD bond angle	0.785
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.500	1.190
High resolution limit [Å]	1.150	1.150
Rmerge	0.106	0.579
Rpim	0.034	0.370
Number of reflections	33230	991
<I/σ(I)>	20.1	1.5
Completeness [%]	81.5	24.7
Redundancy	9.1	2.3
CC(1/2)	1.000	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	292	3.5M Sodium Formate,100mM TRIS pH 8.2