Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.527, 116.527, 130.712 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.570 - 2.470 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pp9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.571 | 2.560 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.179 | |
| Rmeas | 0.184 | |
| Rpim | 0.041 | 0.459 |
| Number of reflections | 37262 | 3669 |
| <I/σ(I)> | 14.8 | 2 |
| Completeness [%] | 99.9 | |
| Redundancy | 19.8 | |
| CC(1/2) | 1.000 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 100 mM Bis-Tris pH 6.4, 200 mM Ammonium Sulfate, and 22% PEG3350 |
