7M0I

Crystal structure of a human metapneumovirus subtype B2 trimeric fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	114.530, 128.100, 431.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.690 - 2.810
R-factor	0.2558
Rwork	0.254
R-free	0.29080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l1x
RMSD bond length	0.013
RMSD bond angle	1.764
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.690	2.911
High resolution limit [Å]	2.810	2.811
Number of reflections	155097	1294
<I/σ(I)>	6.5	0.77
Completeness [%]	75.6
Redundancy	14.9	14.5
CC(1/2)	0.973	0.227

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris pH 8.5, 2.0 M Ammonium sulfate