7LZR
Crystal structure of the BCL6 BTB domain in complex with OICR-10256
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.363, 73.055, 64.421 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 31.300 - 1.340 |
| R-factor | 0.1366 |
| Rwork | 0.135 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r28 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.050 | 1.343 |
| High resolution limit [Å] | 1.339 | 1.339 |
| Rmerge | 0.041 | 0.610 |
| Rmeas | 0.046 | 0.691 |
| Rpim | 0.022 | 0.332 |
| Number of reflections | 108129 | 1112 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 293 | 10% PEG 6000, 0.1M MES pH 4.6, 100 mM AmSO4, 10% glycerol, 10% DMSO |
