7LYF

Dengue virus RNA promoter stem-loop A fused with tRNA-scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-07
Detector	RAYONIX MX-300
Wavelength(s)	0.9785
Spacegroup name	I 2 2 2
Unit cell lengths	30.924, 137.558, 336.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.688 - 3.400
R-factor	0.2299
Rwork	0.226
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tn1 5ew4
RMSD bond length	0.007
RMSD bond angle	1.281
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.366	3.370
Number of reflections	10308	10308
<I/σ(I)>	37
Completeness [%]	97.2
Redundancy	7.1
CC(1/2)	0.991	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM sodium cacodylate pH 6.9, 9% (w/v) polyethylene glycol 8000, 200 mM KCl, and 100 mM magnesium acetate