7LXR
Kinesin-like protein at 61F (Klp61f) bound to AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-29 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 42.692, 50.813, 93.344 |
Unit cell angles | 78.53, 77.87, 73.20 |
Refinement procedure
Resolution | 48.110 - 1.744 |
Rwork | 0.185 |
R-free | 0.22380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hqd |
RMSD bond length | 0.013 |
RMSD bond angle | 1.953 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.110 | 1.840 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.057 | 0.272 |
Number of reflections | 67986 | 9356 |
<I/σ(I)> | 12.56 | 2.5 |
Completeness [%] | 91.8 | |
Redundancy | 3.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 10% ethylene glycol, 50mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (sodium HEPES) pH 7.5, 180mM NaI and 15-17% polyethylene glycol MW 3,350 |