7LWB

Crystal Structure of phospho-Rab8a with the RH2 domain (117-165) of RILPL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	62.773, 68.430, 128.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.260 - 1.900
R-factor	0.2209
Rwork	0.219
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rir
RMSD bond length	0.014
RMSD bond angle	1.329
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.260	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.057	4.301
Rmeas		4.610
Rpim	0.021	1.620
Number of reflections	21414	1964
<I/σ(I)>	16.79	0.35
Completeness [%]	96.0	90.3
Redundancy	8.6	7.9
CC(1/2)	0.999	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	288	150mM DL-Malic acid, 20% PEG3350