7LW8

Human Exonuclease 5 crystal structure in complex with a ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1158
Spacegroup name	P 2 21 21
Unit cell lengths	51.170, 83.980, 96.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.178 - 2.880
R-factor	0.2073
Rwork	0.205
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lw7
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.180	48.180	3.040
High resolution limit [Å]	2.880	9.110	2.880
Rmerge	0.436	0.137	2.043
Rmeas	0.455	0.145	2.126
Rpim	0.126	0.045	0.585
Number of reflections	9904	370	1407
<I/σ(I)>	6.4	16.2	1.6
Completeness [%]	100.0	99.6	100
Redundancy	12.8	10.9	13
CC(1/2)	0.986	0.994	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	16% PEG 3350 and 0.2 M lithium acetate