7LW7

Human Exonuclease 5 crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1158
Spacegroup name	P 2 21 21
Unit cell lengths	50.818, 83.836, 95.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.983 - 2.500
R-factor	0.1842
Rwork	0.182
R-free	0.22450
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.528
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.983	47.983	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.246	0.046	1.263
Rmeas	0.267	0.051	1.373
Rpim	0.104	0.021	0.531
Number of reflections	14791	375	1617
<I/σ(I)>	8.2	30	1.6
Completeness [%]	100.0	99.7	100
Redundancy	6.4	5.4	6.5
CC(1/2)	0.988	0.998	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350 and 0.2 M lithium acetate