7LV3
Crystal structure of human protein kinase G (PKG) R-C complex in inhibited state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2018-08-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.832, 112.824, 150.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 2.496 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.103 | 0.100 |
Number of reflections | 66393 | 6568 |
<I/σ(I)> | 20.93 | |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | |
CC(1/2) | 0.999 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 120 mM ethylene glycol, 100 mM Bicine (pH 8.5), 20 % w/v PEG 8,000 |