7LUO

N-terminus of Skp2 bound to Cyclin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 4 21 2
Unit cell lengths	109.738, 109.738, 152.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.980 - 3.170
R-factor	0.2357
Rwork	0.234
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fin
Data reduction software	XDS (Jun 1, 2017)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	109.740	109.740	3.390
High resolution limit [Å]	3.170	8.970	3.170
Rmerge	0.203	0.036	1.631
Rmeas	0.217	0.039	1.737
Rpim	0.073	0.014	0.581
Total number of observations	142882	6203	26428
Number of reflections	16322	817	2909
<I/σ(I)>	10.3	42.4	1.4
Completeness [%]	99.8	98.9	100
Redundancy	8.8	7.6	9.1
CC(1/2)	0.996	0.999	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	1.7 M Sodium-potassium phosphate