7LUF

HSV1 polymerase ternary complex with dsDNA and PNU-183792

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	181.300, 181.300, 233.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.330 - 3.500
R-factor	0.2268
Rwork	0.226
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gv9
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	181.300	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	94834	4777
<I/σ(I)>	8
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.997	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283.15	0.1 M Na HEPES pH 6.8 and 0.4-0.7 M disodium tartrate