7LSK

Structure of HIV-1 Reverse Transcriptase in complex with DNA, L-dTTP, and CA(2+) ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	89.942, 132.467, 139.342
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	70.760 - 2.700
R-factor	0.2163
Rwork	0.215
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d3g
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.760	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.337	3.782
Rmeas	0.343	3.858
Rpim	0.067	0.762
Number of reflections	88920	4541
<I/σ(I)>	9.7	1.5
Completeness [%]	100.0	100
Redundancy	25.9	25.4
CC(1/2)	0.997	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	10-12% PEG 8000, 50 MM BISTRIS-PROPANE PH 7.2, 50 MM AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE