7LRF
Netrin-1 in complex with SOS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-05-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54192 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.105, 80.152, 241.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.357 - 3.210 |
| R-factor | 0.2291 |
| Rwork | 0.226 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ove |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.360 | 3.430 |
| High resolution limit [Å] | 3.208 | 3.210 |
| Rmerge | 0.138 | |
| Rmeas | 0.172 | |
| Rpim | 0.102 | |
| Number of reflections | 24533 | 2351 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.3 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.995 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293.15 | 20% PEG 8000, 100 mM CHES |
