7LQN
Glucosamine-6-phosphate Deaminase from H. influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.405, 144.300, 131.143 |
| Unit cell angles | 90.00, 92.07, 90.00 |
Refinement procedure
| Resolution | 47.490 - 3.000 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dea |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | PHENIX (1.19) |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 3.060 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.170 | 0.530 |
| Rmeas | 0.203 | 0.629 |
| Rpim | 0.109 | 0.336 |
| Total number of observations | 256858 | |
| Number of reflections | 76608 | 4516 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.972 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 2 % w/v Tasciminate pH 5.0, 0.1 M Sodium Citrate tribasic pH 5.6, 16 % PEG 3350 with 0.1 M Strontium Chloride. 6H2O as additive |
