7LMR

Structure of full-length human lambda-6A light chain JTO in complex with stabilizer 63 [4-methyl-3-(morpholinomethyl)-7-(2-phenylpropoxy)-2H-chromen-2-one]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	63.742, 81.938, 95.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.960 - 2.090
R-factor	0.2046
Rwork	0.202
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mg5
RMSD bond length	0.009
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	2.200
High resolution limit [Å]	2.090	2.090
Rpim	0.034	0.251
Number of reflections	30392	4309
<I/σ(I)>	12.3	2.9
Completeness [%]	99.7	98.1
Redundancy	7.2	7.6
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	20% PEG 3350 and 0.25 M NH4H2PO4 at 23 degrees C