7LML

Receptor for Advanced Glycation End Products VC1 domain in complex with 3-(3-(((3-(4-Carboxyphenoxy)benzyl)oxy)methyl)phenyl)-1H-indole-2-carboxylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 62
Unit cell lengths	102.478, 102.478, 102.158
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.700 - 2.150
R-factor	0.2131
Rwork	0.212
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.004
RMSD bond angle	0.768
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	2.532
High resolution limit [Å]	2.150	2.150
Rmeas	0.072	0.124
Rpim	0.026	0.044
Number of reflections	32950	12707
<I/σ(I)>	16.2	3.66
Completeness [%]	99.3	99.1
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	2.5 M NaOAc (pH 7.5)