7LKY

Crystal Structure of PHF1 Tudor domain in complex with a peptidomimetic ligand UNC6641

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-08-17
Detector	RDI CMOS_8M
Wavelength(s)	1.28
Spacegroup name	P 1
Unit cell lengths	43.394, 45.345, 64.442
Unit cell angles	90.02, 95.19, 90.07

Refinement procedure

Resolution	43.220 - 1.850
R-factor	0.2372
Rwork	0.233
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hcz
Data reduction software	autoXDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.220	1.920
High resolution limit [Å]	1.850	1.850
Rmerge		0.334
Number of reflections	37960	2652
<I/σ(I)>	8.85
Completeness [%]	90.9
Redundancy	1.9
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	0.01 M ZnCl2, 0.1 M MES pH 6.0 and 20% PEG 6000