7LKI

The crystal structure of Epitope III of HCV envelop protein E2 in complex with antibody 1H8

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	201.683, 42.793, 110.824
Unit cell angles	90.00, 109.53, 90.00

Refinement procedure

Resolution	35.699 - 2.000
Rwork	0.245
R-free	0.29440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zea
RMSD bond length	0.006
RMSD bond angle	1.513
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.091	0.050	0.511
Rmeas	0.110	0.059	0.660
Rpim	0.060	0.031	0.412
Total number of observations	212753
Number of reflections	73746	4047	2699
<I/σ(I)>	8.1
Completeness [%]	95.3	99.2	70.2
Redundancy	2.9	3.5	2
CC(1/2)		0.995	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	20% PEG3350 0.1 M citrate pH 5.5