7LK9
Crystal structure of SARS-CoV-2 RBD-targeting antibody COV107-23 HC + COVD21-C8 LC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 |
Unit cell lengths | 73.297, 78.383, 91.626 |
Unit cell angles | 100.12, 112.09, 92.80 |
Refinement procedure
Resolution | 40.190 - 3.400 |
R-factor | 0.2776 |
Rwork | 0.276 |
R-free | 0.31150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xc2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 7.550 | 3.300 |
Rmerge | 0.299 | 0.089 | 0.709 |
Rmeas | 0.354 | 0.104 | 0.851 |
Rpim | 0.188 | 0.054 | 0.464 |
Total number of observations | 79514 | ||
Number of reflections | 24563 | 2043 | 1970 |
<I/σ(I)> | 2.1 | ||
Completeness [%] | 88.0 | 87.5 | 83.4 |
Redundancy | 3.2 | 3.7 | 2.6 |
CC(1/2) | 0.992 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293.15 | 0.1 M of sodium citrate pH 4, 1 M lithium chloride, and 20% (w/v) polyethylene glycol 6000 |