7LJB
Human TRAAK K+ channel mutant G158D in a K+ bound conductive conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2019-06-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.140, 137.310, 96.300 |
Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
Resolution | 48.030 - 2.970 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wff |
RMSD bond length | 0.009 |
RMSD bond angle | 1.579 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.030 | 3.232 |
High resolution limit [Å] | 2.970 | 2.970 |
Rmerge | 0.207 | 2.023 |
Rmeas | 2.109 | |
Rpim | 0.597 | |
Number of reflections | 31745 | 1984 |
<I/σ(I)> | 8.5 | 1.234 |
Completeness [%] | 93.6 | 21.23 |
Redundancy | 11.6 | 6.22 |
CC(1/2) | 0.999 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 50mM Tris pH 8.8, 64-200 mM CaCl2, 27-33%(vol/vol) PEG400. |