7LH9

Crystal structure of BRPF2 PWWP domain in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	40.410, 216.113, 54.037
Unit cell angles	90.00, 109.42, 90.00

Refinement procedure

Resolution	41.640 - 2.600
R-factor	0.227
Rwork	0.225
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z02
RMSD bond length	0.006
RMSD bond angle	1.355
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.094	0.051	0.862
Rmeas	0.110	0.059	1.019
Rpim	0.057	0.029	0.534
Total number of observations	92761
Number of reflections	25975	1354	1339
<I/σ(I)>	7.5
Completeness [%]	97.1	98.9	98.5
Redundancy	3.6	3.8	3.5
CC(1/2)		0.997	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG 3350, 0.2M Magnesium Acetate