7LFC

Structure of importin a3 bound to p50 NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.953
Spacegroup name	P 1 21 1
Unit cell lengths	47.650, 59.070, 86.090
Unit cell angles	90.00, 96.34, 90.00

Refinement procedure

Resolution	14.930 - 2.100
R-factor	0.1887
Rwork	0.187
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tbk
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.044	0.412
Number of reflections	27642	2009
<I/σ(I)>	11.6	2.4
Completeness [%]	98.8	87.7
Redundancy	5.5
CC(1/2)	0.998	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.2M potassium thiocyanate, 20% PEG3350, 0.1 M BIS-TRIS pH 6.5