7LF7

Fab 6D12 bound to ApoL1 NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2016-11-03
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 43
Unit cell lengths	131.103, 131.103, 87.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.458 - 2.026
R-factor	0.19
Rwork	0.189
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fvd
RMSD bond length	0.003
RMSD bond angle	0.771
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.704	2.152
High resolution limit [Å]	2.026	2.027
Rmerge	0.073	0.956
Rmeas	0.083	1.250
Rpim	0.051	0.797
Number of reflections	76896	3846
<I/σ(I)>	12.8	1.5
Completeness [%]	95.1	64.4
Redundancy	4.5	4.5
CC(1/2)	0.998	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	20% PEG 3350, 0.1M Ammonium Sulfate, 0.1M MES pH 6, 14mM sodium cholate