7LEW

Crystal structure of UBE2G2 in complex with the UBE2G2-binding region of AUP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	49.739, 58.226, 63.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.819 - 1.736
R-factor	0.1934
Rwork	0.192
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h8k
RMSD bond length	0.011
RMSD bond angle	1.116
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.800
High resolution limit [Å]	1.736	3.750	1.736
Rmerge	0.034	0.018	0.502
Number of reflections	19573	2094	1917
<I/σ(I)>	16.5
Completeness [%]	99.6	98.8	99.5
Redundancy	5.4	5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	PEG 4000, ammonium acetate etc.