7LCF
Dihydrodipicolinate synthase (DHDPS) from C.jejuni, with pyruvate bound in the active site and L-lysine bound at the allosteric site in C121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.020, 85.890, 200.220 |
| Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
| Resolution | 45.980 - 2.690 |
| R-factor | 0.2294 |
| Rwork | 0.227 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ly8 |
| Data reduction software | HKL-3000 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.980 | 2.786 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.110 | 0.503 |
| Rmeas | 0.712 | |
| Rpim | 0.110 | 0.503 |
| Number of reflections | 108564 | 10831 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 98.8 | 99.66 |
| Redundancy | 15 | |
| CC(1/2) | 0.984 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.4 | 288.15 | 0.7 M Magnesium acetate, 10 % PEG 8000, 0.1 M Sodium acetate (pH 7.4), 60 mM L-Lysine |
