7LAW
crystal structure of GITR complex with GITR-L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 172.661, 172.661, 172.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 122.090 - 2.752 |
| R-factor | 0.2293 |
| Rwork | 0.229 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1m 3wvt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (18-SEP-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.090 | 2.981 |
| High resolution limit [Å] | 2.750 | 2.930 |
| Rpim | 0.036 | 0.380 |
| Number of reflections | 21565 | 916 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 97.0 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% PEG 1500, 0.1 M citric acid, and 4% (v/v) 2-methyl-2,4-pentanediol |
