7LA5
Structure of human GGT1 in complex with Lnt1-172 compound.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.223, 118.855, 101.457 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.930 - 2.070 |
R-factor | 0.2252 |
Rwork | 0.223 |
R-free | 0.28090 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4z9o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.489 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.8.0073) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.130 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.129 | 0.781 |
Rmeas | 0.142 | 0.876 |
Rpim | 0.058 | 0.383 |
Number of reflections | 36505 | 2507 |
<I/σ(I)> | 11.25 | 1.88 |
Completeness [%] | 96.9 | 94.05 |
Redundancy | 5.4 | 4.1 |
CC(1/2) | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20-25%PEG3350, 0.1M ammonium cloride, 0.1M sodium cacodilate pH 6.0 |