7LA5
Structure of human GGT1 in complex with Lnt1-172 compound.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.223, 118.855, 101.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.930 - 2.070 |
| R-factor | 0.2252 |
| Rwork | 0.223 |
| R-free | 0.28090 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4z9o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.8.0073) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.130 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.129 | 0.781 |
| Rmeas | 0.142 | 0.876 |
| Rpim | 0.058 | 0.383 |
| Number of reflections | 36505 | 2507 |
| <I/σ(I)> | 11.25 | 1.88 |
| Completeness [%] | 96.9 | 94.05 |
| Redundancy | 5.4 | 4.1 |
| CC(1/2) | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20-25%PEG3350, 0.1M ammonium cloride, 0.1M sodium cacodilate pH 6.0 |
