7L8Q
Crystal structure of human GPX4-U46C with oxidized Cys-46
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.757, 69.035, 35.678 |
Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
Resolution | 34.520 - 1.480 |
R-factor | 0.157 |
Rwork | 0.154 |
R-free | 0.18210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7l8k |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.040 | 1.500 |
High resolution limit [Å] | 1.480 | 1.480 |
Number of reflections | 23698 | 1049 |
<I/σ(I)> | 16 | |
Completeness [%] | 99.0 | 87.4 |
Redundancy | 3.3 | 2.8 |
CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5 | 293 | 0.1 M potassium thiocyanate, 0.1 M sodium acetate, and 20% (w/v) PEG 8000 |