7L4X

YTH Domain of Human YTHDC1 with dsDNA Comprising Single N6mA joined by two six-bp DNA duplexes in P3221 Crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	56.744, 56.744, 108.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.490 - 1.790
R-factor	0.2003
Rwork	0.199
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wea
RMSD bond length	0.004
RMSD bond angle	0.706
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.490	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.096	1.439
Rpim	0.024	0.537
Number of reflections	19738	1934
<I/σ(I)>	17.5	2.7
Completeness [%]	99.8
Redundancy	17.5	7.4
CC(1/2)		0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	292	30% v/v 2-propanol, 0.1M sodium citrate tribasic dihydrate, 0.1M sodium cacodylate pH 4.6