7L18
Crystal structure of a tandem deletion mutant of rat NADPH-cytochrome P450 reductase
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1999-11-22 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.058, 114.821, 120.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.787 - 2.542 |
| Rwork | 0.181 |
| R-free | 0.25380 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1amo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.8.0267) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.263 | |
| Number of reflections | 43215 | 1879 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 91.9 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 18% PEG4000, 150 mM HEPES, pH 7.0, 150 mM sodium acetate, protein, two-fold molar excess of NADP+ |
