7L0G
Monobody 12VC1 Bound to HRAS(G12C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.834, 62.596, 123.404 |
| Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
| Resolution | 46.840 - 2.540 |
| R-factor | 0.19633 |
| Rwork | 0.195 |
| R-free | 0.22852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g0n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.344 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.240 | 6.080 | 2.240 |
| Rmerge | 0.174 | 0.118 | 0.900 |
| Rmeas | 0.197 | 0.134 | 1.014 |
| Rpim | 0.091 | 0.064 | 0.462 |
| Total number of observations | 247542 | ||
| Number of reflections | 51349 | 2681 | 2519 |
| <I/σ(I)> | 3.7 | ||
| Completeness [%] | 98.6 | 98.5 | 99.1 |
| Redundancy | 4.8 | 4.5 | 4.6 |
| CC(1/2) | 0.988 | 0.737 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350 |
