7KXW

Crystal structure of DCLK1-KD in complex with DCLK1-IN-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	143.950, 61.714, 65.312
Unit cell angles	90.00, 103.04, 90.00

Refinement procedure

Resolution	44.288 - 3.002
R-factor	0.2031
Rwork	0.200
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jzj
Data scaling software	Aimless (0.5.32)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.290	44.290	3.180
High resolution limit [Å]	3.000	9.010	3.000
Rmerge	0.156	0.057	0.775
Rmeas	0.169	0.062	0.839
Rpim	0.064	0.023	0.316
Total number of observations	76277	2942	11337
Number of reflections	11200	439	1669
<I/σ(I)>	11.4	25.6	3.6
Completeness [%]	98.8	99.2	93
Redundancy	6.8	6.7	6.8
CC(1/2)	0.996	0.997	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.25	298	PEG400, ammonium sulfphate, Hepes