7KX8

Crystal structure of DCLK1-Cter in complex with FMF-03-055-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	65.920, 62.430, 72.080
Unit cell angles	90.00, 96.07, 90.00

Refinement procedure

Resolution	71.676 - 3.100
R-factor	0.1957
Rwork	0.193
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jzj
RMSD bond length	0.003
RMSD bond angle	0.658
Data scaling software	Aimless (0.5.8)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.680	71.680	3.310
High resolution limit [Å]	3.100	8.770	3.100
Rmerge	0.236	0.055	0.927
Rmeas	0.263	0.061	1.032
Rpim	0.115	0.026	0.444
Total number of observations	50639	2388	9282
Number of reflections	10702	512	1926
<I/σ(I)>	7.3	16.9	2.6
Completeness [%]	99.5	99.7	99.8
Redundancy	4.7	4.7	4.8
CC(1/2)	0.980	0.998	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.25	298	PEG400, ammonium sulfphate, Hepes