7KTK

DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Ground State Ternary Complex, 50 mM Mg2+ (90min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.109, 61.775, 118.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.194 - 1.419
R-factor	0.1441
Rwork	0.143
R-free	0.16840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.440
High resolution limit [Å]	1.419	3.850	1.420
Rmerge	0.089	0.073	0.953
Rmeas	0.098	0.080	1.054
Rpim	0.039	0.033	0.444
Number of reflections	83788	4482	4113
<I/σ(I)>	11.4
Completeness [%]	99.9	99.4	99.9
Redundancy	6.1	5.8	5.4
CC(1/2)		0.991	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000