7KTE

DNA Polymerase Mu, 8-oxodGTP:Ct Reaction State Ternary Complex, 50 mM Mg2+ (90min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.940, 68.523, 110.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.958 - 1.479
R-factor	0.1578
Rwork	0.157
R-free	0.17740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.479	4.020	1.480
Rmerge	0.136	0.122	0.886
Rmeas	0.149	0.134	0.974
Rpim	0.060	0.056	0.397
Number of reflections	76692	4107	3758
<I/σ(I)>	11.1
Completeness [%]	99.9	99.4	99.9
Redundancy	6.2	5.8	5.9
CC(1/2)		0.973	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000