7KSU

DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mn2+ (4min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-17
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.985, 68.882, 109.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.823 - 1.650
R-factor	0.174
Rwork	0.173
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.143	0.156	0.976
Rmeas	0.156	0.170	1.062
Rpim	0.060	0.066	0.410
Total number of observations	370613
Number of reflections	54904	2968	2696
<I/σ(I)>	9.6
Completeness [%]	99.4	98.9	99.5
Redundancy	6.8	6.4	6.2
CC(1/2)		0.968	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG 4000