7KQ7

Crystal structure of IL21R in complex with an antibody Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	230
Detector technology	PIXEL
Collection date	2010-05-05
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	155.482, 246.501, 55.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.216 - 2.203
R-factor	0.2161
Rwork	0.215
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house structure
RMSD bond length	0.009
RMSD bond angle	1.191
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.580
Number of reflections	47436	1437
<I/σ(I)>	18.8
Completeness [%]	87.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	10% PEG MME 5000 and 0.1M MES, pH 6.5