7KP4
Crystal structure of human claudin-4 in complex with Clostridium perfringens enterotoxin C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-18 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.360, 116.230, 118.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.810 - 3.370 |
| R-factor | 0.294 |
| Rwork | 0.294 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ov2 poly(ALA) and displaced cCpE from 6ov2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS (20180427) |
| Data scaling software | XSCALE (20180427) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.030 | 3.449 |
| High resolution limit [Å] | 3.365 | 3.365 |
| Rmeas | 0.063 | 5.940 |
| Number of reflections | 26037 | 1756 |
| <I/σ(I)> | 8.11 | 0.29 |
| Completeness [%] | 98.0 | 88.8 |
| Redundancy | 2.9 | 2.56 |
| CC(1/2) | 1.000 | 0.139 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 100 mM DL-malic acid + MES + Tris base (1:2:2), pH 6.0, 25% PEG1500 |
