7KMR
Crystal structure analysis of human KRAS mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 78.400, 78.400, 55.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.570 - 1.510 |
| R-factor | 0.1633 |
| Rwork | 0.162 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tar |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.570 | 55.610 | 1.540 |
| High resolution limit [Å] | 1.510 | 4.100 | 1.510 |
| Rmeas | 0.167 | 0.039 | 2.284 |
| Rpim | 0.054 | 0.013 | 0.705 |
| Total number of observations | 311129 | 15737 | 15850 |
| Number of reflections | 31274 | 1689 | 1523 |
| <I/σ(I)> | 14.4 | 53.4 | 1.1 |
| Completeness [%] | 99.9 | 100 | 99.9 |
| Redundancy | 9.9 | 9.3 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 1.5 M sodium malonate, 0.1 M HEPES, pH 7.5 |
