7KMG
LY-CoV555 neutralizing antibody against SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.317, 280.364, 68.909 |
| Unit cell angles | 90.00, 99.62, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.160 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yla |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.290 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.040 | 0.444 |
| Number of reflections | 77441 | 9083 |
| <I/σ(I)> | 6.2 | 1.5 |
| Completeness [%] | 91.2 | 82.9 |
| Redundancy | 3.1 | 2.7 |
| CC(1/2) | 0.999 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 20% PEG 10000, sodium acetate pH 4.6 |
