7KLZ

Structure of SPOP MATH domain in complex with a Geminin peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9786
Spacegroup name	P 41 21 2
Unit cell lengths	103.060, 103.060, 131.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.990 - 3.400
R-factor	0.213
Rwork	0.210
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f8g
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	3.520
High resolution limit [Å]	3.390	3.390
Rmerge	0.096	1.270
Number of reflections	10264	985
<I/σ(I)>	15.24
Completeness [%]	99.8
Redundancy	37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	One microliter of the protein complex in 20 mM Tris-HCl, pH 7.6, 50 mM NaCl and 5 mM dithiothreitol was mixed with on microliter of 0.2 M ammonium citrate dibasic and 20% (W/V) PEG 3350