7KL5

Structure of Calmodulin Bound to the Cardiac Ryanodine Receptor (RyR2) at Residues: Phe4246 to Val4271

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	33.169, 62.403, 43.190
Unit cell angles	90.00, 107.49, 90.00

Refinement procedure

Resolution	41.192 - 1.650
Rwork	0.209
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bcx
RMSD bond length	0.015
RMSD bond angle	1.804
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180319)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.400	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.050	0.865
Rmeas	0.060	1.030
Rpim	0.032
Number of reflections	19805	3226
<I/σ(I)>	9.3	1.5
Completeness [%]	97.9	96.7
Redundancy	3.4	3.4
CC(1/2)	0.997	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.2M sodium acetate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000