7KKH

P1A4 Fab in complex with ARS1620

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1158
Spacegroup name	C 1 2 1
Unit cell lengths	103.782, 71.302, 86.793
Unit cell angles	90.00, 90.66, 90.00

Refinement procedure

Resolution	58.770 - 2.000
R-factor	0.1862
Rwork	0.185
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gzn 2b0s
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.770	2.072
High resolution limit [Å]	2.000	2.000
Number of reflections	42846	4212
<I/σ(I)>	7.75
Completeness [%]	99.4
Redundancy	10.2
CC(1/2)	0.996	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	0.1 M HEPES pH 7.5, 70 % MPD