7KKF

Crystal Structure of S. cerevisiae Ess1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.917
Spacegroup name	C 1 2 1
Unit cell lengths	110.098, 57.366, 69.334
Unit cell angles	90.00, 96.92, 90.00

Refinement procedure

Resolution	29.210 - 2.400
R-factor	0.2712
Rwork	0.269
R-free	0.30650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yw5
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.392	5.170	2.400
Rmerge	0.099	0.070	0.712
Rmeas	0.107	0.076	0.768
Rpim	0.040	0.030	0.286
Number of reflections	16821	1721	1694
<I/σ(I)>	10.8
Completeness [%]	99.8	98.8	100
Redundancy	7	6.6	7
CC(1/2)		0.996	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris, pH 7.7 and 21% (w/v) PEG 8000