7KJB
Crystal structure of the EphA2 S897E/S901E mutant intracellular KD-SAM domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-04-07 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 94.456, 94.456, 99.646 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.530 - 2.800 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pdo 3kka |
RMSD bond length | 0.003 |
RMSD bond angle | 0.499 |
Data reduction software | XDS (VERSION Oct 15, 2015) |
Data scaling software | Aimless (version 0.5.23) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.530 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.285 | 1.956 |
Rmeas | 0.313 | 2.153 |
Rpim | 0.130 | 0.895 |
Number of reflections | 13051 | 1871 |
<I/σ(I)> | 10.2 | 1.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.9 | 11 |
CC(1/2) | 0.992 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 4.5% Tacsimate, 0.09 M HEPES pH 7.0, 9% PEG-MME 5K, 0.1 M CsCl |